4000-520-616
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主营:主营: AMV-RT,PCR, Enzymes, Trehalose, NASBA, Lyophilized
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4000-520-616
当前位置: 首页 > 产品中心 > RNA_polymerase > 甘油/T7G-15000/15000 u中的Lifesci/T7 RNA聚合酶
商品详细甘油/T7G-15000/15000 u中的Lifesci/T7 RNA聚合酶
甘油/T7G-15000/15000 u中的Lifesci/T7 RNA聚合酶
甘油/T7G-15000/15000 u中的Lifesci/T7 RNA聚合酶
商品编号: T7G-15000
品牌: Lifesci
市场价: ¥2820.00
美元价: 1692.00
产地: 美国(厂家直采)
公司:
产品分类: RNA聚合酶
公司分类: RNA_polymerase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductDescription

T7RNAPolymerasecatalysesthesynthesisofRNAinthepresenceofdouble-strandedDNAcontainingaT7promotersequence.ItisisolatedfromanE.colitransformedbyaplasmidcontainingtheT7RNAPolymerasegene.Thisenzymeisprovidedinglycerol-basedstoragebuffercontaining:20mMpotassiumphosphatepH7.5,100mMNaCl,1.0mMEDTA,10mMDTT,0.2%TritonX-100and50%glycerol.Alternatively,itisalsoprovidedinthesamebuffercontaining1.0Mtrehaloseinsteadofglycerol(suitableforlyophilization).Concentration70,000units/mL.

Pleaseinquireforcustomconcentrationsandbulkquantities.

Applications:

PreparationoffluorescentorrADIoactivelylabeledRNAprobes
PreparationoflargeRNAtranscriptsforanalysisorinvitrotranslation
AmplificationofRNA(inconjuctionwithAMVRTandRibonucleaseH.)
Preparationofanti-senseRNA
Preparationofribozymes
PreparationofmRNAprecursorforsplicing
PreparationofdsRNAforRNAinterferenceorsilencing
PreparationofsubstrateinRNaseprotectionassays
PreparationoftemplateforgenomicDNAsequencing
PreparationofRNAforstudiesofRNAsecondarystructureandRNA-proteininteractions

Reagentssupplied:

5XReactionBufferforT7RNAPolymerase

1XReactionBufferConditions:

40mMTrisHCl,pH7.7
6mMMgCl2
10mMDTT
2mMSpermidine

Tobesupplementedwith:

0.5mMATP,CTP,GTPandUTP
DNAtemplatewithT7promoter
(20nMofalinearizedplasmidDNAor40ug/mLofa3Kbplasmid)
Incubateat37ºC

UnitDefinition:

Oneunitisdefinedastheamountofenzymethatwillincorporate3nmolofUridinetriphosphateintoacid-insolubleforminonehourat37ºCunderstandardassayconditions:40mMTrisHCl,pH7.5,30mMMgCl2,20mMDTT,0.4mMGTP,CTP,ATP,UTPand[3H]-UTP,20µg/mLnon-linearizedplasmidDNA,50µg/mLBSA.

GeneralNotes:

DithiothreitolisrequiredforT7RNAPolymeraseactivity.ThislABIlecomponentofthereactionbuffermayberestoredbysupplementingreactionswithafinalconcentrationof10mMDTT.
HigheryieldsofRNAmaybeobtainedbyincreasingtheconcentrationofNTP’stoasmuchas4mM.
Caremustbetakenthatthetotalsaltconcentrationinthereactiondoesnotexceed50mM,sincethisenzymeissensitivetosaltconcentrationsexceedingthisamount.

QualityControlofT7RNAPolymerase

1.)DNaseActivity:One-halfµgofHaefragmentsofPhiX-174DNAisincubatedat37ºCwith175unitsofT7RNAPolymerasefor3hours,andthenelectrophoresedinanativeagarosegelsimultaneouslywithcontrolpositiveDNase1reactions.Nomorethantheequivalentof1.25X10E-4unitofDNase1isdetected.

2.)RibonucleaseActivity:OnemicrogramofanRNALadderisincubatedfor2hourswith280unitsofT7RNAPolymerase,andthenelectrophoresedinanativeagarosegelsimultaneouslywithcontrolpositiveRNase1Areactions.Nomorethantheequivalentof8.0X10E-8unitofRNase1Aisdetected.

3.)SpecificActivity:ThespecificactivityoftheT7RNAPolymeraseisnolessthan400,000unitspermg.

References:

Sambrook,J.,Fritsch,E.F.,andManiatis,T.(1989)MolecularCloning:ALaboratoryManual,(2ndEd.),10.27-10.37
Sambrook,J.,Fritsch,E.F.,andManiatis,T.(1989)MolecularCloning:ALaboratoryManual,(2ndEd.),18.82-18.84

品牌介绍
Lifesci-NASBA套件和NASBA产品基于核酸序列的扩增产品–生命科学高级技术公司在制造NASBA产品和NASBA试剂方面已经领先20多年。我们的NASBA(基于核酸序列的扩增产品)产品系列包括单个产品和方便使用的套件。浏览我们精选的NASBA相关产品,找到最适合您的产品。由于受到广大客户的需求,Life Sciences Advanced Technologies现在以一包提供完整的NASBA湿试剂盒和NASBA冻干试剂盒,作为您研发的完整便捷的解决方案。按默认顺序排序  显示12产品 缺货SKU:AMV LRT(海藻糖中的冻干AMV逆转录酶)$ 230.00细节SKU:D-PDG(用于LEM的PDGT稀释剂)$ 7.50 – $ 15.00选择选项细节SKU:DRB(LRB的反应重组缓冲区)$ 7.50 – $ 15.00选择选项细节SKU:LEM(用于RNA扩增的冻干酶混合物(NASBA))$ 45.00 – $ 90.00选择选项细节SKU:LRB(用于RNA扩增的冻干反应缓冲液(NASBA))$ 37.00 – $ 74.00选择选项细节SKU:NEC-1-24(NASBA酶鸡尾酒(湿混))$ 159.50添加到购物车细节